Effect of alpha‐Synuclein on the binding of [18F]MK‐6240 and [18F]AV‐1451

نویسندگان

چکیده

Background Binding in alpha-synuclein containing brain regions have been described for both Flortaucipir ([18F]AV-1451) and [18F]MK-6240. Here we assessed whether alpha-Synuclein constitutes a target these tau imaging agents. Method We used autoradiographic dot blot to validate specific evaluate off-target interactions of [18F]MK-6240 [18F]AV-1451 using sarkosyl-insoluble extracts from PSP, CBD, PiD, AD cortical tissue which contain fibrillar forms tau. Tissue concentrations are orders magnitude lower than NFTs leading us use recombinant PFFs potential interactions. Briefly, 1:100 dilutions each preparation were incubated with 82 fmol mass 30uL total volume. Samples 90 minutes at 37°C. Reactions terminated by pipetting the solution triplicate onto glass fiber filter held within 96-well dot-blot aspiration device samples immobilized. Unbound ligand was washed three times ice cold PBS. The filters transferred cassette exposure film activity photostimulated luminescence units per mm2 calculated ImageJ software v.1.8.0. Result Disintegrations corrected decay normalized cerebellar ligands. demonstrated high specificity, binding subject trypsin treatment. similar native trypsinized extracts, while also showing overlapping profiles human but not mouse PFFs. Conclusion binds as it does AD-tau PHFs. Future experiments should assess patient derived aggregates off [18F]AV-1451.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

the effect of task complexity on lexical complexity and grammatical accuracy of efl learners’ argumentative writing

بر اساس فرضیه شناخت رابینسون (2001 و 2003 و 2005) و مدل ظرفیت توجه محدود اسکهان (1998)، این تحقیق تاثیر پیچیدگی تکلیف را بر پیچیدگی واژگان و صحت گرامری نوشتار مباحثه ای 60 نفر از دانشجویان زبان انگلیسی بررسی کرد. میزان پیچیدگی تکلیف از طریق فاکتورهای پراکندگی-منابع تعیین شد. همه ی شرکت کنندگان به صورت نیمه تصادفی به یکی از سه گروه: (1) گروه موضوع، (2) گروه موضوع + اندیشه و (3) گروه موضوع + اندی...

15 صفحه اول

the effect of taftan pozzolan on the compressive strength of concrete in the environmental conditions of oman sea (chabahar port)

cement is an essential ingredient in the concrete buildings. for production of cement considerable amount of fossil fuel and electrical energy is consumed. on the other hand for generating one tone of portland cement, nearly one ton of carbon dioxide is released. it shows that 7 percent of the total released carbon dioxide in the world relates to the cement industry. considering ecological issu...

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Alzheimers & Dementia

سال: 2023

ISSN: ['1552-5260', '1552-5279']

DOI: https://doi.org/10.1002/alz.065616